Compositions containing amines and use thereof

ABSTRACT

The present invention features compositions comprising at least one compound of the formula I or formula II: 
                         
wherein R1, R2, R3, R4, and R5 independently, are selected from the group consisting of hydrogen, C 1 -C 6  alkyl, C 1 -C 6  hydroxyalkyl, or a cosmetically acceptable salt thereof, and the use thereof.

CROSS-REFERENCE TO RELATED APPLICATIONS

The present application is a divisional application of U.S. patentapplication Ser. No. 11/223,032, priority of which is hereby claimed,filed on Sep. 9, 2005 now U.S. Pat. No. 8,221,046 which is acontinuation-in-part of application Ser. No. 11/066,362, priority ofwhich is hereby claimed, filed Feb. 25, 2005 now abandoned, which arehereby incorporated by reference in their entirety.

BACKGROUND OF THE INVENTION

N,N,N′,N′-tetrakis(2-hydroxypropyl)ethylenediamine has been disclosedfor use as a catalytic agent. For example, PCT Patent Application No.WO/0170132 describes the use ofN,N,N′,N′-tetrakis(2-hydroxypropyl)ethylenediamine as catalytic agent topolymerize micropsheres prior to injection into the skin. Injection ofmicrospheres into the skin acts to augment skin contour deficienciessuch as wrinkles. The use of tertiary amines, such asN,N,N′,N′-tetrakis(2-hydroxypropyl)ethylenediamine, as chelating agentsto prevent the reduction of the salicylic acid is described in U.S. Pat.No. 4,822,604.

The application of N,N,N′,N′-tetrakis(2-hydroxypropyl)ethylenediamine incosmetic products has been described in European Patent No. 0023978whereby the development of carcinogenic nitrosamines in cosmetic andtoiletry products which contain triethanolamine is avoided by usingN,N,N′,N′-tetrakis(2-hydroxypropyl)ethylenediamine to neutralize acidicformulations. U.S. Pat. No. 4,749,507 describes the use ofN,N,N′,N′-tetrakis(2-hydroxypropyl)ethylenediamine, for removing hairdyes from hair and skin and U.S. Pat. No. 3,916,008 describes the use ofesters of ethylene diamine are useful as hypocholesterolaemic agents inanimals and man. Bhide M V et al., Immunopharmacol. 1985; 7(3):303-312reported that treating macrophages in culture withN,N,N′,N′-tetrakis(2-hydroxypropyl)ethylenediamine resulted in anincreased macrophage activation, a pro-inflammatory response.

The present invention relates to the unexpected discovery that certainamine compounds, such asN,N,N′,N′-tetrakis(2-hydroxypropyl)ethylenediamine, are topicallyeffective for treating signs of aging and/or non-acne irritation andinflammation of skin. Furthermore, it was also unexpectedly discoveredthat such amine compounds also have the ability to potentiate theactivity of other cosmetically active agents.

SUMMARY OF THE INVENTION

In one aspect, the present invention features a method of (i) treatingat least one sign of aging on the skin selected from the groupconsisting of enhancing the elasticity of said skin, enhancing thefirmness of said skin, and reducing the appearance of wrinkles orcellulite on the skin, and/or (ii) treating non-acne inflammation ofskin by administering to skin in need of such treatment a compositioncomprising at least one compound of the formula I or formula IIdescribed below.

In another aspect, the present invention features a product containing(a) a composition including at least one compound of the formula I orformula II described below and (b) instructions directing the user toapply said composition to skin in order to (i) treat at least one signof aging on the skin selected from the group consisting of enhancing theelasticity of said skin, enhancing the firmness of said skin, andreducing the appearance of wrinkles on the skin or (ii) treat non-acneinflammation of skin.

In another aspect, the present invention also features a method ofpromoting a composition including at least one compound of the formula Ior formula II described below, wherein said method comprises directingthe user to apply said composition to skin in order to (i) treat atleast one sign of aging on the skin selected from the group consistingof enhancing the elasticity of said skin, enhancing the firmness of saidskin, and reducing the appearance of wrinkles on the skin and/or (ii)treat non-acne inflammation of skin.

In one embodiment, the present invention features a method ofpotentiating the activity of a cosmetically active agent byadministering to skin in need of treatment with such active agent acomposition comprising at least one compound of the formula I or formulaII described below and such active agent.

In one embodiment, the present inventions features a compositioncomprising: (a) at least one compound of the formula I or formula IIdescribed below and (b) at least one member selected from the groupconsisting of a feverfew extract, a soy extract, an anti-inflammatoryagent, or retinol.

Other features and advantages of the present invention will be apparentfrom the detailed description of the invention and from the claims.

DETAILED DESCRIPTION OF THE INVENTION

It is believed that one skilled in the art can, based upon thedescription herein, utilize the present invention to its fullest extent.The following specific embodiments are to be construed as merelyillustrative, and not limitative of the remainder of the disclosure inany way whatsoever.

Unless defined otherwise, all technical and scientific terms used hereinhave the same meaning as commonly understood by one of ordinary skill inthe art to which the invention belongs. Also, all publications, patentapplications, patents, and other references mentioned herein areincorporated by reference. Unless otherwise indicated, a percentagerefers to a percentage by weight (i.e., % (W/W)).

DEFINITIONS

What is meant by “treat or treating a sign of aging on the skin” isreducing the appearance of wrinkles on the skin and/or enhancing thefirmness or elasticity of the skin, including but not limited to,treating sagging, lax and loose skin or tightening skin.

What is meant by “potentiating the activity of a cosmetically activeagent” is promoting, enhancing or sustaining the activity of othercosmetically active agents, such as anti-inflammatory active agents orretinoids, such as retinol and retinoic acid.

What is meant by “treating non-acne inflammation of skin” is reducing orpreventing redness or inflammation of skin not caused by acne.

What is meant by an “anti-inflammatory agent” is an agent that has andIC50 of less than or equal to 100 μg/ml for Interleukin-2,Interferon-gamma, tissue necrosis factor-alpha, and/or granulocytemacrophage stimulating factor in the assay set forth below in Example 6.Examples of anti-inflammatory agents include, but are not limited tonon-steroidal anti-inflammatory agents (e.g., ibuprofen, aspirin,naproxen, and acetaminophen), anesthetics (e.g., benzocaine, lidocaine,and pramoxine hydrochloride), corticosteroids (e.g., betamethasonedipropionate, betamethasone valerate, clobetasol propionate, diflorasonediacetate, halobetasol propionate, amcinonide, desoximetasone,fluocinonide, fluocinolone acetonide, halcinonide, triamcinoloneacetate, hydrocortisone, hydrocortisone valerate, hydrocortisonebutyrate, aclometasone dipropionte, flurandrenolide, mometasone furoate,methylprednisolone acetate), Vitamin D compounds (e.g. calcipotriene),and various plant extracts (e.g., feverfew and soybean extracts).

What is meant by a “product” is a product in finished packaged form. Inone embodiment, the package is a container such as a plastic, metal orglass tube or jar containing the composition. The product may furthercontain additional packaging such as a plastic or cardboard box forstoring such container. In one embodiment, the product containsinstructions directing the user to administer the composition to thetissue to enhance its firmness or elasticity. Such instructions may beprinted on the container, label insert, or on any additional packaging.

What is meant by “contract a skin cell” is to reduce the length of atleast one dimension of the skin cell. Examples of skin cells include,but are not limited to, keratinocytes.

What is meant by “promoting” is promoting, advertising, or marketing.Examples of promoting include, but are not limited to, written, visual,or verbal statements made on the product or in stores, magazines,newspaper, radio, television, internet, and the like.

For promoting the contraction of skin cells, examples of such statementsinclude, but are not limited to, “contracts skin cells,” “contractskeratinocytes,” “shrinks skin cells,” and “shrinks keratinocytes”.Examples of such visual statements include digital images, pictures,drawings, or movies of skin cells depicting contracted cells and/or thecontraction of cells (e.g., showing a reduction in the length of atleast one dimension of the skin cell). In one embodiment, the skin cellsare of the of the upper epidermis.

For promoting the treatment of signs of aging, examples of suchstatements include, but are not limited to, “enhances skin elasticity,”“improving visible and tactilely perceptible manifestations of theskin,” “increases skin elasticity or firmness,” “restores skinelasticity,” “treats sagging or lax skin,” “reduces the appearance ofcellulite,” “lifts the skin,” and “lifts the face,” “firms the skin,”“firms the face,” “younger skin,” “restores youthful firmness”,“improves facial contours,” and “makes skin look younger.”

For promoting the treatment of inflammation, examples of such statementsinclude, but are not limited to, “reduces inflammation,” “reducesredness,” and “reduces the appearance of inflammation.”

As used herein, the term “wrinkle” includes fine line, fine wrinkles,coarse wrinkles, cellulite, scars, and stretch marks. Examples ofwrinkles include, but are not limited to, fine lines around the eyes(e.g., “crow's feet”), forehead and cheek wrinkles, frown-lines, andlaugh-lines around the mouth.

As used herein, “administering to skin in need of such treatment” meanscontacting (e.g., by use of the hands or an applicator such, but notlimited to, a wipe, tube, roller, spray, or patch) the area of skin inneed such treatment or an area of skin proximate to the area of skin inneed of such treatment (e.g., to contract an area of skin proximate tothe area of need of treatment, thereby tightening the area of skin inneed of such treatment).

As used herein, “composition” means a composition suitable foradministration to the skin.

As used herein, “cosmetically-acceptable” means that the ingredientswhich the term describes are suitable for use in contact with the skinwithout undue toxicity, incompatibility, instability, irritation,allergic response, and the like.

As used herein, “safe and effective amount” means an amount of thecompound, carrier, or of the composition sufficient to induce anenhancement in tissue elasticity, but low enough to avoid serious sideeffects. The safe and effective amount of the compounds or compositionwill vary with the area being treated, the age, health and skin type ofthe end user, the duration and nature of the treatment, the specificcompound or composition employed, the particular cosmetically-acceptablecarrier utilized, and like factors.

Compounds

The compositions of the present invention contain at least one compoundof the formula I or formula II:

wherein R1, R2, R3, R4, and R5 independently, are selected from thegroup consisting of hydrogen, C₁-C₆ alkyl, and C₁-C₆ hydroxyalkyl; or acosmetically-acceptable salt thereof.

In one embodiment, the compositions of the present invention contain atleast one compound of the formula I and R1, R2, R3, and R4 are selectedfrom the group consisting of C₁-C₃ alkyl and C₁-C₃ alkanol. In a furtherembodiment, at least one of R1, R2, R3, and R4 of formula I is a C₂-C₃alkanol group bearing at least one hydroxyl group.

Examples of compounds of formula I include, but are not limited to,N,N,N′,N′-Tetrakis(2-hydroxypropyl)ethylenediamine (THPED),N,N,N′,N′-Tetrakis(2-hydroxyethyl)ethylene diamine (THEED),N,N,N′,N′-tetramethylethylene diamine (TEMED) (the structures of whichare set forth below), enantiomers thereof, or diastereoisomers thereof,or cosmetically-acceptable salts thereof.

The synthesis of N,N,N′,N′-tetrakis(2-hydroxypropyl)ethylenediamine fromthe reaction of ethylenediamine with of propylene oxide is described inU.S. Pat. No. 2,697,118.

The compounds of the present invention may also be present in the formof cosmetically acceptable salts. For use in medicine, the salts of thecompounds of this invention refer to non-toxic “cosmetically acceptablesalts,” cosmetically acceptable acidic/anionic or basic/cationic salts.Cosmetically acceptable acidic/anionic salts include, and are notlimited to acetate, benzenesulfonate, benzoate, bicarbonate, bitartrate,bromide, calcium edetate, camsylate, carbonate, chloride, citrate,dihydrochloride, edetate, edisylate, estolate, esylate, fumarate,glyceptate, gluconate, glutamate, glycollylarsanilate, hexylresorcinate,hydrabamine, hydrobromide, hydrochloride, hydroxynaphthoate, iodide,isethionate, lactate, lactobionate, malate, maleate, mandelate,mesylate, methylbromide, methylnitrate, methylsulfate, mucate,napsylate, nitrate, pamoate, pantothenate, phosphate/diphospate,polygalacturonate, salicylate, stearate, subacetate, succinate, sulfate,tannate, tartrate, teoclate, tosylate and triethiodide. Cosmeticallyacceptable basic/cationic salts include, and are not limited toaluminum, benzathine, calcium, chloroprocaine, choline, diethanolamine,ethylenediamine, lithium, magnesium, meglumine, potassium, procaine,sodium and zinc. Other salts may, however, be useful in the preparationof compounds according to this invention or of their cosmeticallyacceptable salts. Organic or inorganic acids also include, and are notlimited to, hydriodic, perchloric, sulfuric, phosphoric, propionic,glycolic, methanesulfonic, hydroxyethanesulfonic, oxalic,2-naphthalenesulfonic, p-toluenesulfonic, cyclohexanesulfamic,saccharinic or trifluoroacetic acid.

In one embodiment, the compositions of present invention contain fromabout 0.01% to about 10% by weight of such compound, such as from about0.1% to about 5% by weight of such compound, such as from about 0.5% toabout 3% by weight of such compound.

Compositions

The compositions useful in the present invention involve formulationssuitable for administering to the target tissues, such as mammalian skinsuch as human skin. In one embodiment, the composition contains a safeand effective amount of (i) compounds of the present invention and (ii)a cosmetically-acceptable carrier. In one embodiment, thecosmetically-acceptable carrier is from about 50% to abut 99.99%, byweight, of the composition (e.g., from about 80% to about 99%, byweight, of the composition).

The compositions may be made into a wide variety of product types thatinclude but are not limited to solutions, suspensions, lotions, creams,gels, sticks, sprays, ointments, cleansing liquid washes and solid bars,shampoos and hair conditioners, pastes, foams, powders, mousses, shavingcreams, wipes, patches, nail lacquers, wound dressing and adhesivebandages, hydrogels, film-forming products, facial and skin masks,make-up such as foundations, mascaras, and lipsticks, and the like.These product types may contain several types of cosmetically—acceptablecarriers including, but not limited to solutions, suspensions, emulsionssuch as microemulsions and nanoemulsions, gels, solids and liposomes.The following are non-limitative examples of such carriers. Othercarriers can be formulated by those of ordinary skill in the art.

The compositions useful in the present invention can be formulated assolutions. Solutions typically include an aqueous or organic solvent(e.g., from about 50% to about 99.99% or from about 90% to about 99% ofa cosmetically acceptable aqueous or organic solvent). Examples ofsuitable organic solvents include: propylene glycol, polyethylene glycol(200-600), polypropylene glycol (425-2025), glycerol, 1,2,4-butanetriol,sorbitol esters, 1,2,6-hexanetriol, ethanol, and mixtures thereof.

A lotion can be made from such a solution. Lotions typically containfrom about 1% to about 20% (e.g., from about 5% to about 10%) of anemollient(s) and from about 50% to about 90% (e.g., from about 60% toabout 80%) of water. As used herein, “emollients” refer to materialsused for the prevention or relief of dryness, as well as for theprotection of the skin or hair. Examples of emollients include, but arenot limited to, those set forth in the International Cosmetic IngredientDictionary and Handbook, eds. Wenninger and McEwen, pp. 1656-61, 1626,and 1654-55 (The Cosmetic, Toiletry, and Fragrance Assoc., Washington,D.C., 7^(th) Edition, 1997) (hereinafter “ICI Handbook”).

Another type of product that may be formulated from a solution is acream. A cream typically contains from about 5% to about 50% (e.g., fromabout 10% to about 20%) of an emollient(s) and from about 45% to about85% (e.g., from about 50% to about 75%) of water.

Yet another type of product that may be formulated from a solution is anointment. An ointment may contain a simple base of animal, vegetable, orsynthetic oils or semi-solid hydrocarbons. An ointment may contain fromabout 2% to about 10% of an emollient(s) plus from about 0.1% to about2% of a thickening agent(s). Examples of thickening agents include, butare not limited to, those set forth in the ICI Handbook pp. 1693-1697.

The compositions useful in the present invention can also be formulatedas emulsions. If the carrier is an emulsion, from about 1% to about 10%(e.g., from about 2% to about 5%) of the carrier contains anemulsifier(s). Emulsifiers may be nonionic, anionic or cationic.Examples of emulsifiers include, but are not limited to, those set forthin the ICI Handbook, pp. 1673-1686.

Lotions and creams can be formulated as emulsions. Typically suchlotions contain from 0.5% to about 5% of an emulsifier(s), while suchcreams would typically contain from about 1% to about 20% (e.g., fromabout 5% to about 10%) of an emollient(s); from about 20% to about 80%(e.g., from 30% to about 70%) of water; and from about 1% to about 10%(e.g., from about 2% to about 5%) of an emulsifier(s).

Single emulsion skin care preparations, such as lotions and creams, ofthe oil-in-water type and water-in-oil type are well-known in the artand are useful in the subject invention. Multiphase emulsioncompositions, such as the water-in-oil-in-water type or theoil-in-water-in-oil type, are also useful in the subject invention. Ingeneral, such single or multiphase emulsions contain water, emollients,and emulsifiers as essential ingredients.

The compositions of this invention can also be formulated as a gel(e.g., an aqueous, alcohol, alcohol/water, or oil gel using a suitablegelling agent(s)). Suitable gelling agents for aqueous and/or alcoholicgels include, but are not limited to, natural gums, acrylic acid andacrylate polymers and copolymers, and cellulose derivatives (e.g.,hydroxymethyl cellulose and hydroxypropyl cellulose). Suitable gellingagents for oils (such as mineral oil) include, but are not limited to,hydrogenated butylene/ethylene/styrene copolymer and hydrogenatedethylene/propylene/styrene copolymer. Such gels typically containsbetween about 0.1% and 5%, by weight, of such gelling agents.

The compositions of the present invention can also be formulated into asolid formulation (e.g., a wax-based stick, soap bar composition,powder, and wipe containing powder).

The compositions useful in the subject invention may contain, inaddition to the aforementioned components, a wide variety of additionaloil-soluble materials and/or water-soluble materials conventionally usedin compositions for use on skin at their art-established levels.

Additional Cosmetically Active Agents

In one embodiment, the composition further contains another cosmeticallyactive agent in addition to the above compounds. What is meant by a“cosmetically active agent” is a compound (e.g., a synthetic compound ora compound isolated from a natural source, or a natural extractcontaining a mixture of compounds) that has a cosmetic or therapeuticeffect on the tissue, including, but not limiting to, lightening agents,darkening agents such as self-tanning agents, anti-acne agents, shinecontrol agents, anti-microbial agents such as anti-yeast agents,anti-fungal, and anti-bacterial agents, anti-inflammatory agents,anti-parasite agents, external analgesics, sunscreens, photoprotectors,antioxidants, keratolytic agents, detergents/surfactants, moisturizers,nutrients, vitamins, energy enhancers, anti-perspiration agents,astringents, deodorants, hair removers, hair growth enhancing agents,hair growth delaying agents, firming agents, anti-callous agents, agentsfor skin conditioning, anti-cellulite agents, fluorides, andodor-control agents such as odor masking or pH-changing agents.

In one embodiment, the cosmetically active agent is selected from, butnot limited to, the group consisting of hydroxy acids, benzoyl peroxide,D-panthenol, octyl methoxycinnimate, titanium dioxide, octyl salicylate,homosalate, avobenzone, carotenoids, free radical scavengers, spintraps, retinoids and retinoid precursors such as retinol and retinylpalmitate, ceramides, polyunsaturated fatty acids, essential fattyacids, enzymes, enzyme inhibitors, minerals, hormones such as estrogens,steroids such as hydrocortisone, 2-dimethylaminoethanol, copper saltssuch as copper chloride, peptides containing copper such asCu:Gly-His-Lys, coenzyme Q10, amino acids such a proline, vitamins,lactobionic acid, acetyl-coenzyme A, niacin, riboflavin, thiamin,ribose, electron transporters such as NADH and FADH2, and otherbotanical extracts such as aloe vera, Feverfew, and Soy, and derivativesand mixtures thereof. The cosmetically active agent will typically bepresent in the composition of the invention in an amount of from about0.001% to about 20% by weight of the composition, e.g., about 0.005% toabout 10% such as about 0.01% to about 5%.

Examples of vitamins include, but are not limited to, vitamin A, vitaminBs such as vitamin B3, vitamin B5, and vitamin B12, vitamin C, vitaminK, vitamin E such as alpha, gamma or delta-tocopherol, and derivativesand mixtures thereof.

Examples of hydroxy acids include, but are not limited, to glycolicacid, lactic acid, malic acid, salicylic acid, citric acid, and tartaricacid.

Examples of antioxidants include, but are not limited to, water-solubleantioxidants such as sulfhydryl compounds and their derivatives (e.g.,sodium metabisulfite and N-acetyl-cysteine), lipoic acid anddihydrolipoic acid, resveratrol, lactoferrin, and ascorbic acid andascorbic acid derivatives (e.g., ascorbyl palmitate and ascorbylpolypeptide). Oil-soluble antioxidants suitable for use in thecompositions of this invention include, but are not limited to,butylated hydroxytoluene, retinoids (e.g., retinol and retinylpalmitate), different types of tocopherols (e.g., alpha-, gamma-, anddelta-tocopherols and their esters such as acetate) and their mixtures,tocotrienols, and ubiquinone. Natural extracts containing antioxidantssuitable for use in the compositions of this invention, include, but notlimited to, extracts containing flavonoids, isoflavonoids, and theirderivatives such as genistein and diadzein (e.g., such as Soy and Cloverextracts, extracts containing resveratrol and the like. Examples of suchnatural extracts include grape seed, green tea, pine bark, and propolis.

The irritation mitigation effects of the compounds of formula I and IIcan help mitigate the skin irritation effects of certain irritatingcosmetically active agents. Examples of such skin-irritatingcosmetically active agents include, but not limiting to, retinoid andits derivatives, benzoyl peroxide, retinoids, and alpha-hydroxy acids.Examples of retinoid include, but are not limited to, retinol, retinoicacid, retinyl palmitate, retinyl acetate, retinal, and retinylpropionate. Examples of alpha-hydroxy acids include, but are not limitedto, lactic acid and glycolic acid.

Plant Extract

In one embodiment, the compositions of present invention further containa plant extract. What is meant by a “plant extract” is a blend ofcompounds isolated from a plant. Such compounds may be isolated from oneor more part of the plant (e.g., the whole plant, flower, seed, root,rhizome, stem, fruit and/or leaf of the plant) by physically removing apiece of such plant, such as grinding a flower of the plant. Suchcompounds may also be isolated from the plant by using extractionprocedures well known in the art (e.g., the use of organic solvents suchas lower C₁-C₈ alcohols, C₁-C₈ alkyl polyols, C₁-C₈ alkyl ketones, C₁-C₈alkyl ethers, acetic acid C₁-C₈ alkyl esters, and chloroform, and/orinorganic solvents such as water, inorganic acids such as hydrochloricacid, and inorganic bases such as sodium hydroxide).

In one embodiment, the plant extract (e.g., a feverfew extract or asoybean extract) is present in the composition in an amount from about0.001% to about 20% by weight, in particular in an amount from about0.1% to about 10% by weight of the composition. Unless stated otherwise,the weight of the extract refers to the dry weight of the extract.

Feverfew Extract

In one embodiment, the compositions of present invention further containa feverfew extract. What is meant by a “feverfew extract” is a blend ofcompounds isolated from a feverfew plant. Examples of such compounds,include, but are not limited to, apigenin-7-glucoside,apigenin-7-glucuronide, 1-β-hydroxyarbusculin,6-hydroxykaempferol-3,7-4′-trimethylether (Tanetin),6-hydroxykaempferol-3,7-dimethyl ether, 8-β-reynosin, 10-epicanin,ascorbic acid, beta-carotene, calcium, chromium, chrysanthemolide,chrysanthemomin, chrysarten-A, chrsyarten-c, chrysoeriol-7-glucuronide,cobalt, cosmosiin, epoxyartemorin, luteolin-7-glucoside,luteolin-7-glucuronide, mangnoliolide, parthenolide,quercetagentin-3,7,3′-trimethylether, quercetagetin-3′7-dimethylether,reynosin, tanaparthin, tanaparthin-1α, 4α-epoxide,tanaparthin-1β,4β-epoxide, β-costunolide, 3-β-hydroxy-parthenolide, and3,7,3′-trimethoxyquercetagetin. The α-unsaturated γ-lactones present inthe feverfew plant, such as parthenolide, are known to cause theallergic reactions. Therefore, in one embodiment, the feverfew extractis substantially free of the allergy causing α-unsaturated γ-lactones.The preparation of feverfew extract that is substantially free ofparthenolide is disclosed in Example 1 in U.S. Patent Application No.20040105905.

Soybean Extract

In one embodiment, the compositions of present invention further containa soybean extract. What is meant by a “soybean extract” is a blend ofcompounds isolated from soybean. The soybean extract may contain only aportion of the soybean (e.g., an extract of the soybean such as a lipidreduced soybean powder or filtered soymilk) or may contain the entiresoybean (e.g., a ground powder of the soybean). The soybean extract maybe in the form of a fluid (e.g., soymilk) or a solid (e.g., a soybeanpowder or soymilk powder).

The soybean extract may be soybean powder. Soybean powder may be made bygrinding dry soybeans. The soybean powder may be lyophilized. Soymilkand soymilk powder are also useful soybean extracts. Soymilk is acombination of solids derived from soybeans and water, the mixture ofwhich has some or all of the insoluble constituents filtered off.Soymilk powder is evaporated soymilk, which in one embodiment, is in alyophilized or spray-dried form. Procedures for manufacturing soymilkinclude, but are not limited to, the following three procedures. First,soymilk may be made by placing soybeans into water to allow them toabsorb the water. The swelled beans are then ground and additional wateris then added. The mixture may then be filtered to remove any insolubleresidue. Second, soymilk may also be prepared from soybean powder.Soybean powder is thoroughly mixed with water (e.g., for at least onehour), which may then be followed by a filtration process to removeinsoluble residues. Third, soymilk can also be reconstituted fromsoymilk powder by adding water. The soymilk may comprise from about 1%to about 50%, by weight (e.g., from about 5% to about 20%, by weight) ofsolids from the soybean.

The known active ingredients of soybeans include, but not limiting to,isoflavones, phytoestrogens, genistein, daidzein, glycitein, saponins,and phytosterols. The soybean extracts useful in this invention may beproduced from all soybean species, regardless of their geographicorigin, sun exposure, harvest time and the like. However, specificstrains, geographic origins or growth conditions might be preferred. Forexample, but not limiting to, soybean strains particularly rich in itsSoybean Trypsin Inhibitor (STI) content or in isoflavone content, orgrowth conditions that result in STI or isoflavone enrichment in thebean, might be preferred.

In one embodiment, the soybean extract is a non-denatured soybeanextract. “Denaturation” is defined in the Bantam Medical Dictionary(1990 edition) as “the change in the physical and the physiologicalproperties of a protein, that are brought about by heat, X-rays orchemicals. These changes include loss of activity (in the case ofenzymes) and loss (or alteration) of antigenicity (in the case ofantigens)”. What is meant by “non-denatured plant extract” is a productextracted or derived from a plant in which the processing for thederivation of such plant extract (e.g., the temperature, extractionmedia) did not eliminate its protease inhibitory activity. One suchprotease is trypsin. In one embodiment, the non-denatured state of thesoybean extract of this invention is measured by the presence of anintact soybean trypsin inhibitor (STI) protein, or by its trypsininhibitory activity.

It should be noted that the soybean extracts useful in the compositionsof this invention may have a distinctive odor. If necessary, the odor ofthe extracts may be reduced by using soybean products derived fromspecific strains of soybeans known to produce reduced-odor, including,but not limited to, lipoxygenase-2-deficient beans and those havingmodified sugar profile, and the like. A process to reduce oxygen levelsin the formulation may also reduce the odor. Various masking agents orfragrances may also be used to mask the odor. One way to make soymilk isto soak the soybeans in deionized or purified water for several hours,and grind them after they were fully hydrated, with the addition ofsmall quantities of water. The grinding process allows the soybean milkto be extracted. After collection, the soybean milk may be filtered toremove any residual parts of the bean husk.

The soymilk used in the formulations described below can be freshsoymilk as described above, or may be made from soybean powder andwater. The soybean powder is milled from soybeans and may also belyophilized, spray dried, or freeze-dried and the resulting soymilk mayor may not be filtered. Such prepared soymilk may have from about 1 toabout 90% by weight dry soybean powder. Another example is the use ofsoymilk powder, made from lyophilized, spray dried or freeze-driedsoymilk, with the addition of water and finished with or withoutfiltration or homogenization. Other methods of soybean extraction couldalso be used to create the active ingredients in the formulationsdescribed below. For example, the active ingredients could be extractedfrom ground soybeans using ethanol/water mixtures, followed by theremoval of the ethanol from the extract, in such ways that the serineprotease inhibitory activity of the soybean will be retained, andpreferably that the protein STI will remain intact. In one embodiment,the soybean extracts utilized in the present invention have a microbialcontent of less than about 1,000 cfu per gram (such as less than about100 cfu per gram) of the soybean extract.

The soybean extract may be exposed to gamma irradiation. The soybeanextract may be exposed to from about 2 to about 30 kGy of gammairradiation, such as from about 5 and about 10 kGy of gamma irradiation.Such treatment reduces the microbial content of the soybean extract,while maintaining its biological activity (e.g., serine proteaseinhibitory activity). The treatment of soybean extracts with gammairradiation maintains the cosmetic elegance of the composition, such asmaintained natural colors and does not induce significant malodors.

Other anti-microbial processes that also maintain the proteaseinhibitory activity of the soybean extract that can be practiced aloneor in combination with gamma irradiation, include, but are not limitedto, exposure to x-rays, high energy electron or proton beams,ultraviolet radiation, hydrostatic pressure, and addition of chemicalagents possessing antimicrobial activity, and combinations thereof.

Other Materials

Various other materials may also be present in the compositions usefulin the subject invention. These include humectants, proteins andpolypeptides, preservatives and an alkaline agent. Examples of suchagents are disclosed in the ICI Handbook, pp. 1650-1667. Thecompositions of the present invention may also contain chelating agents(e.g., EDTA) and preservatives (e.g., parabens). Examples of suitablepreservatives and chelating agents are listed in pp. 1626 and 1654-55 ofthe ICI Handbook. In addition, the compositions useful herein cancontain conventional cosmetic adjuvants, such as colorants such as dyesand pigments, opacifiers (e.g., titanium dioxide), and fragrances.

Mineral Water

The compositions of the present invention may be prepared using amineral water, for example mineral water that has been naturallymineralized such as Evian® Mineral Water (Evian, France). In oneembodiment, the mineral water has a mineralization of at least about 200mg/L (e.g., from about 300 mg/L to about 1000 mg/L). In one embodiment,the mineral water contains at least about 10 mg/L of calcium and/or atleast about 5 mg/L of magnesium.

Use

The composition according to the invention can be used to treat avariety of skin conditions, such as the signs of aging and a variety ofnon-acne inflammatory disorders such as those caused by both externaland inherent conditions. Examples of such inflammatory disorders whichmay be treated by topical use of the compositions of this inventioninclude, but are not limited to the following: arthritis, contactdermatitis, atopic dermatitis, psoriasis, seborrheic dermatitis, eczema,allergic dermatitis, polymorphous light eruptions, inflammatorydermatoses, folliculitis, alopecia, poison ivy, insect bites, irritationinduced by extrinsic factors including, but not limited to, chemicals,trauma, pollutants (such as cigarette smoke) and sun or wind exposure,and secondary conditions resulting from inflammation including but notlimited to xerosis, hyperkeratosis, pruritus, post-inflammatoryhyperpigmentation, scarring and the like.

The composition and formulations containing such compositions of thepresent invention may be prepared using methodology that is well knownby an artisan of ordinary skill.

Example 1 Electric Cell-Substrate Impedance Detection

Electrical changes due to the presence of a cell layer can be used tocalculate cell morphological parameters including the barrier functionof the cell layer (the spacing between the ventral side of the cell andthe substratum) and the cell membrane capacitance (Giaever and Keese,Proceedings of National Academy of Sciences 88, 7896, 1991).

Current flowing between a reference electrode, onto which cells or cellcultures may be attached, and a larger counter electrode using normalculture medium as the electrolyte can be used to detect changes in cellmorphological parameters. In the absence of cells on the electrode, thecurrent flows unrestrained from the surface of the electrodes. In thepresence of cells attached and spread upon the electrode, the currentmust now flow in the spaces under and between the cells, as the cellmembrane act as insulators.

The electrical changes of living, viable cells can be sampled rapidly,in real time, over an extended period of time, and the measurements arethe measurement is non-invasive. An example of this technology is theElectric Cell-substrate Impedance Sensing System (ECIS) available fromApplied Biophysics (Troy, N.Y.). Electrical changes in keratinocytemorphological parameters can be used to identify novel compounds foranti-aging benefits.

Human keratinocytes and Epilife culture media (Cascade Biologics,Portland, Oreg.) were cultured at 37° C. in a humidified atmospheres of5% CO₂/95% air. Electric Cell-substrate Impedance Sensing System (ECIS)electrode arrays (Applied Biophysics, Troy, N.Y.) were coated with 0.01mg/ml Laminin V (Sigma Aldirch, St Louis, Mo.) in sterile PhosphateBuffered Saline (Gibco Life Sciences, San Diego, Calif.). Humankeratinocytes were prepared at a density of 0.125×10⁶ cells/mL inEpilife culture media. Human keratinocytes were plated at 5.0×10⁴cells/electrode well and cultured at 37° C. in a humidified atmospheresof 5% CO₂/95% O₂ for 24-48 hrs. Capacitance changes (in nanofarads, nF)of keratinocytes were measured using a Electric Cell-substrate ImpedanceSensing System (ECIS) Model 1600R (Applied Biophysics, Troy, N.Y.) usingthe method of Wegener and co-workers (Wegener J, Keese C R, Giaever I,Exp Cell Res. 259:158-166, 2000) at a frequency of 40,000 Hz.Capacitance readings are inversely related to the contact area of a cellonto the ECIS electrode array, the greater the cell area contacting theelectrode the smaller the capacitance readings (Wegener J, Keese C R,Giaever I, Exp Cell Res. 259:158-166, 2000). Conversely as a cellcontracts or shrinks, the contact area of the cell onto the ECISelectrode array decreases, resulting in an increase in the capacitancereadings. The change in capacitance readings for each treatment groupswas integrated over 5 hrs as a function of area-under-the-curve (AUC)analysis from approximately 1000 keratinocyte cells. These calculationsare based on the trapezoid theorem. The AUC of the media curve wassubtracted from the compound treated group. A positive AUC differenceindicates keratinocyte contraction, the greater the AUC difference thegreater the amount of keratinocyte contraction. The following compoundswere used in the example:N,N,N′,N′-Tetrakis(2-hydroxypropyl)ethylenediamine (THPED, availablefrom BASF under the tradename “Quadrol” or “Neutrol”) and2-(dimethylamino)ethanol (available from BASF under the tradename“DMAE”). DMAE is a known skin-firming agent. The compounds were dilutedinto Hank's Buffered Salt Solution (HBSS; Gibco Life Sciences, SanDiego, Calif.) and the pH of the solution adjusted to between pH7.2-7.3. The pH of the HBSS without compounds (“Media Alone”) was alsoadjusted to pH 7.2-7.3.

Based on this example, it can be seen from the results on table I, thatDMAE and THPED induces a dose-dependent contraction or shrinkage ofkeratinocyte cell area compared to media treatment alone as measured bykeratinocyte capacitance.

TABLE I Quantization of Electrical Capacitance Contraction of HumanKeratinocytes (Mean ± Std Dev) Concentration (% V/V) Treatment 0% 0.01%0.05% 0.1% Media Alone 15.8 ± .8 Media +  29.2 ± 3.2** 35.1 ± 5.3*  44.5± 9.1* THPED Media + 22.82 ± 4.1** 28.8 ± 5.6** 36.1 ± 5.9* DMAE *= P <0.05 compared to AUC for Media Alone treated group using a pairedt-Test. **= P < 0.1 compared to AUC for Media Alone treated group usinga paired t-Test

Example 2 Contraction of Human Keratinocytes with THPED using Real TimeVisual Microscopy

Human keratinocytes and Epilife culture media (Cascade Biologics,Portland, Oreg.) were cultured at 37° C. in a humidified atmospheres of5% CO₂/95% air. Glass Cover Slides (Applied Biophysics, Troy, N.Y.) werecoated with 0.01 mg/ml Laminin V (Sigma Aldirch, St Louis, Mo.) insterile Phosphate Buffered Saline (Gibco Life Sciences, San Diego,Calif.). Human keratinocytes were prepared at a density of 0.125×10⁶cells/mL in Epilife culture media. Human keratinocytes were plated at3.75×10⁴ cells/well on a 6 well plate containing Laminin coatedcoverslips and cultured at 37° C. in a humidified atmospheres of 5%CO₂/95% O₂ for 48 hrs. Individual coverslips were transferred to a openbath imaging chamber (Warner Instruments, Hamden, Conn.) mounted on aLeica inverted microscope (Leitz DMlL) with an attached CCD camera.Cells were perfused with Hank's Buffered Salt Solution (HBSS; Gibco LifeSciences, San Diego, Calif.) at flow rate of 1 ml/min using aperistaltic pump (Cole Parmer, Vernon Hills, Ill.). DMAE or THPED wasdiluted into Hank's Buffered Salt Solution and the pH of the solutionadjusted to between pH 7.2-7.3. The pH of the HBSS without DMAE or THPED(“Media Alone”) was also adjusted to pH 7.2-7.3. Human keratinocyteswere perfused for 10 minutes with buffer alone to establish basalconditions at which time, buffer containing various concentrations ofDMAE or THPED were perfused onto keratinocytes. Images were collected ofa field of keratinocytes containing 10-20 cells at 0, 10 and 20 minutesof treatment using ImagePro software. Cell area was determined usingScion Image software (Version 4.0.2), and reductions in cell area werereported as the ratio of cell area after treatment to cell area prior totreatment

Based on this example, it was found (as shown in Table II) that DMAE andTHPED induces a dose-dependent contraction or shrinkage of keratinocytecell area as determined by keratinocyte surface area.

TABLE II Quantization of Visual Contraction of Human Keratinocytes withTHPED and DMAE (Mean ± Std Dev) Concentration (% V/V) Treatment 2 Min 10Min 20 Min Media Alone 2.1% ± 3.2%  Media +  4.82% ± 0.68%* 6.22% ±0.95%* DMAE (0.01%) Media + 16.40% ± 2.58%* 18.04% ± 3.09%*  DMAE(0.05%) Media + 23.61% ± 3.03%* 33.83% ± 2.96%*  DMAE (0.1%) Media +32.9% ± 11.3%*  37.9% ± 10.7%* 47.3% ± 12.7%* THPED (0.1%) *= P < 0.05compared to cell area of keratinocytes prior to treatment with THPEDusing a paired t-Test.

Based on this example, it can be seen that DMAE and THPED are able tosignificantly induce a rapid contraction or shrinkage of keratinocytecell area.

Example 3 Reviscometer® RVM 600 Reading in the Upper Inner Arm

The Reviscometer® RVM 600 (Courage and Khazaka, Cologne, Germany)measures the propagation time of an elastic shear pulse in viscoelasticmaterials. As the preferred disposition of the collagen fiberscorresponds to the skin's cleavage line (Lange's lines), the speed ofpropagation of elastic disturbances on the skin will depend strongly onits orientation. Skin sites on the body where the skin is the loosestwould present the strongest orientation effects, e.g. on the upper innerarm, the neck, the thighs and the abdomen based on collagen fiberorientation.

In this study we chose an instrument that allows the determination ofdirectional tension along the surface of the skin. The velocity of sounddepends on the density and tension of the material through which it ispropagating, for example sound travels faster in water than it does inair and faster yet in a solid. Mechanical vibrations propagate fasterthe higher the tension, like a guitar string the higher the tension thehigher the frequency of oscillation after plucking. The probe that comesin contact with the skin of the instrument in question is composed oftwo transducers placed 1.5-2 mm apart and mounted on two independentsupports. Then one transducer generates a motion of small amplitude (<1mm) and the second transducer determines when the disturbance generatedby the first transducer arrives at its location. From this time, we cancalculate the velocity of propagation and, therefore, the tension alongthe skin. In the limit where the motion of the transducer is less than100 microns, the instrument would probably probe the tension in theepidermis and as the motion becomes larger the motion would include thedermis. The instrument used in this study generates a motion that probesthe epidermis and the superficial dermis. The time that it takes theacoustic pulse to go from transmitter to receiver is the measuredparameter called Resonance Running Time (RRT). The RRT depends on thedirectional orientation of the collagen bundles. Readings must beperformed in different angles: 0°, 45°, 90° and 135°. In this studyreadings as function of the angle were taken in increments of 3°;covering an angular field of 100° range. The anisotropy (A) of themeasured parameter, RRTmax/RRTmin, and the full width at half maximum(FWHM) obtained from a Gaussian fit of the RRT as a function of themeasured angle are two new mechanical parameter that change with age.Its ratio A/FWHM is a new mechanical parameter that we can use topredict the subjects age since we obtained a p value of <0.001 for thisratio as a function of age. In this study we will express the skinfirming as a ratio of the Anisotropy before and after productapplication.

Skin viscoelastical measurements were performed as a function thedirection with a Reviscometer® (Model RVM 600, Courage Khazaka, Cologne,Germany). The probe is held perpendicular to the skin surface by ahollow cylindrical holder that is attached to the surface of the skinwith double stick tape (positioning top). The holder has marks along itsperiphery at angular intervals of 45°. In our instrument we modified theprobe-holder assembly by placing a mm scale on the probe and another onthe holder. Then we carried out measurements by rotating the probewithin the holder so that the mm lines of the scale would align witheach other, this corresponded to making measurements every 3° for atotal interval of 100°.

The skin viscoelastical measurements were taken on the upper inner armof 30 subject. Two sites were chosen in each arm, readings were taken asdescribed above before and 45 minutes after product application. In oneof the sites was applied a placebo formulation (no THPED) and in thesecond site a formulation containing 2.5% of THPED (as described inExample 4), was applied. After the gaussian fit, the Anisotropy ratio,before and after product application, was calculated.

It was found that the THPED formulation decreases the skin anisotropy 3fold, as compared to 1.5 fold for the placebo formulation. Using aMinitab® software for the statistical analysis comparing THPEDformulation against placebo, we obtained a p<0.001. This shows thatTHPED treated sites can tight and firm the skin and this effect isstatistically significant.

Compositions containing DMAE were also tested in the same methodology asdescribed above. The upper inner arms of subjects were treated withproducts that contained either 0% DMAE (placebo), 0.5% DMAE, 1% DMAE, 2%DMAE, or 3% DMAE. The anisotropy was measured both before and 35 minutesafter product application. The anisotropy ratio shows a dose responsefor DMAE indicating that the contraction of the keratinocytes, as seenin the Confocal Microscopy example (example 3), can firm and tight theskin as well deliver anti-aging benefits to the skin. Table V shows thefirming effect (anisotropy ratio) for the DMAE as a function of DMAEconcentration in percentage.

TABLE V DMAE Concentration Firming (%) (A_(before)/A_(after)) Placebo1.1 ± 1.1   0.5 6.5 ± 5.8 1 9.5 ± 6.3 2 14.5 ± 4.8  3 20.9 ± 5.0 

Example 4 Topical Composition

The following is a description of the manufacture of a topical lotioncomposition containing THPED. Into a primary glass beaker, 545.60 g ofdeionized water was weighed and heated to 78-80° C. While mixing atmoderate speed, 15.0 g of PVM/MA Decadiene Crosspolymer available fromInternational Specialty Products (Wayne, N.J.) under the tradename“Stabileze QM” was added and mixed until homogenous at 78-80° C. Thebeaker was removed from heat, and 1.0 g of Disodium EDTA available fromDow Chemical (Midland, Mich.) under the tradename “Versene NA”, 7.5 g ofSucrose Cocoate available from Croda (Edison, N.J.) under the tradename“Crodesta SL-40”, 7.5 g of PEG-6 Capric/Caprylic Glycerides availablefrom Croda under the tradename “Glycerox 767,” and 10.0 g of HexyleneGlycol available from Pfaltz & Bauer Chemicals (Waterbury, Conn.) underthe tradename “Hexylene Glycol” were added and mixed until uniform.

In a secondary beaker, 305.0 g of deionized water were weighed into aglass beaker. 25.0 g ofN,N,N′,N′-Tetrakis(2-hydroxypropyl)ethylenediamine available from BASFunder the tradename “Quadrol” or “Neutrol” was added and mixed untiluniform. When the temperature of the primary beaker was cooled to 40° C.or below, the contents of the second beaker were added to the primarybeaker and mixed until uniform.

A buffering solution was prepared in a tertiary beaker by first weighing12.4 g of deionized water into a glass beaker. 8.0 g of Anhydrous CitricAcid “Citric Acid” and 23.0 g of Glycolic Acid (70%) available fromDuPont Chemical under the tradename “Glypure” were then added and mixeduntil uniform. The pH of the mixture in the primary beaker was adjustedto between 7.0-7.5 with Glycolic/Citric/Water mixture added from thetertiary beaker.

Then, 5.0 g of Talc available from Luzenac (Denver, Colo.) under thetradename “Windsor Talc 66” was added to the primary beaker and mixeduntil uniform. 10.0 g of Nylon 12 available from Kobo Products, Inc(South Plainfield, N.J.) under the tradename “SP-10” was added to theprimary beaker and mixed until uniform. 10.0 g of SiliconeQuaternium-13available from Biosil Technologies, Inc. (Paterson, N.J.)under the tradename “Biosil Basics SPQ” was added to the primary beakerand mixed until uniform. 10.0 g of Parabens available from NipaLaboratories, Inc. (Wilmington, Del.) under the tradename “Phenonip” wasadded to the primary beaker and mixed until uniform. Finally, themixture was homogenized for 5 minutes and cooled to 25° C.

Example 5 Topical Composition

A topical formulation of Table VI was manufactured as follows. Into aprimary glass beaker, deionized water, glycerin, hydroxycellulose, andparabens were added. N,N,N′,N′-Tetrakis(2-hydroxypropyl)ethylenediamineavailable from BASF under the tradename “Quadrol” or “Neutrol” was thenadded to the primary glass beaker, mixed until uniform and the pH of themixture was adjusted to between 7.0-7.5 with Glycolic Acid. Followingadjustment of the pH, Chrysantemum Parthenium Extract (Feverfew PowderExtract) was added and mixed until uniform.

Into a secondary glass beaker, Petrolatum, Distearyldimonium Chloride,Cetearyl Alcohol, Isopropyl Palmitate, and Dimethicone were added. Themixture in the secondary glass beaker was heated to 80° C., and mixeduntil uniform. When the temperature of the secondary beaker was cooledto 40° C. or below, the contents of the second beaker were added to theprimary beaker along with Benzyl Alcohol and mixed until uniform.Finally, the mixture was homogenized for 5 minutes and cooled to 25° C.

TABLE VI Trade Name INCI Name % W/W Deionized Water Deionized Water63.68 Glycerine 917 Glycerin 12 Varisoft TA-100 DistearyldimoniumChloride 5 Snow White Petrolatum 4 Petrolatum U.S.P. Procol CS-20-DCetearyl Alcohol and 3 Ceteareth-20 Propal NF Isopropyl Palmitate 3Crodacol C-95 Cetyl Alcohol 2.5 Quadrol Tetrahydroxypropl 2.5Ethylenediamine Glypure 70% Glycolic Acid 1.4 Dow Corning 225Dimethicone 1.25 Benzyl Alcohol Benzyl Alcohol 0.6 Feverfew PowderChrysantemum Parthenium 0.5 Extract (Feverfew) Extract NipastatMethylparaben and 0.3 Butylparaben and Ethylparaben and PropylparabenNatrosol 250 HHR Hydroxyethylcellulose 0.27

Example 6 Immunomodulation of Human Peripheral Blood Lymphocyte CytokineRelease Stimulated with PHA

The ability of various amines to affect the inflammatory responses wasillustrated by its ability to reduce the production of cytokines byhuman lymphocytes stimulated with the T-cell receptor (TCR) activatingagent phytohaemagglutinin (PHA) in the following assay.

Human leukocytes were collected from a healthy adult male vialeukopheresis, and adjusted to a density of 1×10⁶ cells/mL in serum freelymphocyte growth medium (ExVivo-15, Biowhittaker, Walkersville, Md.).PBLs were stimulated with 10 μg/mL PHA in the presence or absence oftest samples following published methods (Hamamoto Y., et al. ExpDermatol 2:231-235, 1993). The following compounds were evaluatedN,N,N′,N′-Tetrakis(2-hydroxypropyl)ethylenediamine (THPED, availablefrom BASF under the tradename “Quadrol” or “Neutral”),N,N,N,N-Tetramethylethylenediamine (TEMED, available from Pfaltz & BauerChemicals (Waterbury, Conn.)), and Diethylenetriamine (available fromSigma-Aldrich) Following a 48 hour incubation at 37° C. with 5% CO₂, thesupernatant was removed and evaluated for cytokine content usingcommercially available multiplex cytokine detection kit. The results aredepicted on Table VII.

TABLE VII TNF IL-2 IFN Compound Dose % inh. % inh. % inh. GMCSF % inh.N,N,N,N- 100 μg/ml 41.8% 42.9% 58.6% 53.1% Tetramethylethylenediamine 10 μg/ml 33.6% 6.6% 36.9% 47.4% (TEMED)Tetrahydroxyethylethylenediamine 100 μg/ml 44.5% 61.8% 49.5% 23.0%(THEED)  10 μg/ml 35.8% 27.7% 23.7% 16.6% N,N,N′,N′- 100 μg/ml 46.1%41.3% 37.9% 15.8% Tetrakis(2-  10 μg/ml 13.3% 10.6% 18.1% 10.3%hydroxypropyl)ethylenediamine Diethylenetriamine 100 μg/ml 3.3% −19.5%−35.0% 51.7%  10 μg/ml 9.9% 4.7% −1.2% 36.6% Tris[2- 100 μg/ml −15.3%2.3% 12.5% −52.8% (isopropylamino)ethyl]amine  10 μg/ml −10.7% −7.7%20.3% −26.4% (where IL = Interleukin-2 (Cytokine); IFN =interferon-gamma; TNF = tissue necrosis factor-alpha; GM-CSF =granulocyte macrophage stimulating factor); and % inh. refers to percentinhibition of the cytokine.

Based on the foregoing, it can be seen that the tested compounds wereable to modulate lymphocyte activation induced by T-cell stimulation.

Example 7 Anti-Inflammatory Activity on Release of UV-InducedPro-inflammatory Mediators on Reconstituted Epidermis

The effect of diamines was evaluated for topical anti-inflammatoryactivity on human epidermal equivalents. Epidermal equivalents (EPI 200HCF), multilayer and differentiated epidermis consisting of normal humanepidermal keratinocytes, were purchased from MatTek (Ashland, Mass.).Upon receipt, epidermal equivalents were incubated for 24 hours at 37°C. in maintenance medium without hydrocortisone. Equivalents weretopically treated (2 mg/cm²) with compounds in 70% ethanol/30% propyleneglycol vehicle at the indicated concentration 2 hours before exposure tosolar ultraviolet light (1000 W-Oriel solar simulator equipped with a1-mm Schott WG 320 filter; UV dose applied: 70 kJ/m² as measured at 360nm). Equivalents were incubated for 24 hours at 37° C. with maintenancemedium then supernatants were analyzed for IL-1A cytokine release usingcommercially available kits (Upstate Biotechnology, Charlottesville,Va.). The results are depicted on Table VIII.

TABLE VIII Mean +/− Std Dev of Percent Inhibition Treatment (Dose, IL-1ARelease of Skin as % w/v) (ng/ml) Inflammation Untreated, No UV 115.7 ±15.2 — UV, Vehicle 320.6 ± 35.6 — Treated UV, Feverfew 159.0 ± 38.3**50.4% (1.0%) UV, Feverfew 287.4 ± 75.4 10.4% (0.5%) UV, THPED (0.5%)357.3 ± 92.1   0% UV, Feverfew 180.0 ± 67.4** 43.8% (0.5%) + THPED(0.5%) **Indicates significant difference from UV, Vehicle treated usinga student's t-Test with significance set at P < 0.05.Thus, THPED was found to significantly enhanced the anti-inflammatoryactivity of Feverfew.

Example 8 Reduction of Methyl Nicotinate-Induced Skin Erythema

Methyl nicotinate (methyl 3-pyridinecarboxylate) is a known vasodilatorcausing an increased cutaneous blood flow upon its application on theskin. See Guy R. H., Arch. Dermatol Res (1982) 273:91-95. In thisexperiment, between 1-5 mM-solution of methyl nicotinate (AldrichChemical, St. Louis, Mo.) was topically applied for 30 sec underocclusion (2.5 cm disk, Hill Top Research Inc, Cincinnati, Ohio) on thevolar forearm of 7 volunteers. Formulation placebo or formulationcontaining Feverfew alone or Feverfew extract plus THPED was topicallyapplied 30 minutes before the methyl nicotinate challenge. Redness wasassessed by diffuse reflectance spectroscopy. See Kollias N, et al.,Photochem Photobiol. (1992) (56):223-227. An Ocean Optics (Dunedin,Fla.) Diode array spectrophotometer connected to a HP laptop computerthrough a USB port was used to control the experiment and to collect andanalyze the spectral data. An optic fiber bundle was used to conduct thelight from the lamp to the skin and transmit the reflectancemeasurements back from the skin to the spectrophotometer. The resultsare depicted in Table IX.

TABLE IX Percent Treatment (Dose, Mean +/− Std Dev of Inhibition of as %w/v) Apparent Hemoglobin Skin Erythema Placebo 0.55 ± 0.18 — Feverfew(1%) 0.39 ± 0.26** 28.9% Feverfew (0.5%) 0.49 ± 0.20 8.7% THPED (2.5%) +0.40 ± 0.21** 26.0% Feverfew (0.5%) **Indicates significant differencefrom Placebo treated using a student's t-Test with significance set at P< 0.05.

These results indicate that THPED potentiated the redness-reducingactivity of Feverfew in a methyl nicotinate-induced human redness model

What is claimed is:
 1. A method of treating non-acne inflammation ofskin, said method comprising administering to skin in need of suchtreatment a composition comprising (a) from about 0.01 to about 10% byweight of at least one compound is selected from diethylenetriamine andtris[2-(isopropylamino)-ethyl]amine, or a cosmetically acceptable saltthereof; and (b) from about 0.001 to about 20% by weight of feverfew. 2.A method of claim 1, wherein said composition comprises from about 0.005to about 10% by weight of feverfew.
 3. A method of claim 1, wherein saidcomposition comprises from about 0.01 to about 5% by weight of feverfew.4. A composition comprising: (a) from about 0.01 to about 10% by weightof at least one compound: is selected from diethylenetriamine andtris[2-(isopropylamino)-ethyl]amine, an enantiomer thereof, or adiastereoisomer thereof, or a cosmetically acceptable salt thereof or acosmetically acceptable salt thereof; and (b) from about 0.001 to about20% by weight of feverfew.
 5. A composition of claim 4, wherein saidcomposition comprises from about 0.005 to about 10% by weight offeverfew.
 6. A composition according to claim 5, comprising from about0.01 to about 5% of feverfew.
 7. A composition of claim 4, wherein R1,R2, R3, and R4, independently, are selected from the group consisting ofC₁-C₃ alkyl and C₁-C₃ alkanol.
 8. A composition of claim 4, wherein saidcomposition further comprises at least one member selected from thegroup consisting of a feverfew extract or a soy extract.